@article{Younas_Saba_Bukhari_Naeem_2011, title={Significance of Bone Marrow Histology in the Diagnosis of Acute Myeloid Leukemia}, volume={17}, url={https://www.annalskemu.org/journal/index.php/annals/article/view/260}, DOI={10.21649/akemu.v17i1.260}, abstractNote={Background:  Acute Myeloid Leukemia (AML) is a heterogeneous disease. The precise diagnosis requires a careful morphological examination of a well pre-pared bone marrow aspirate along with flow cytometry and genetic analysis wherever required. Traditionally, bone marrow biopsy has not been considered an essen-tial diagnostic modality for AML. The aim of this stu-dy was to assess the diagnostic as well as prognostic significance of bone marrow histology in patient with acute myeloid leukemia.<br />Materials and Methods:  Forty (40) patients of AML underwent a bone marrow examination including an aspirate and a trephine biopsy. Air dried films of peri-pheral blood and aspirates were fixed in methanol and stained with Giemsa. The following cytochemical stai-ns were also applied: PAS, Myeloperoxidase, Non specific esterase, Chloracetate Esterase and Acid Phos-phatase, and SBB. Bone marrow biopsy specimens were obtained from post superior iliac crest with a manual trephine and were processed in plastic after decalcification.<br /><br />Results:  In all the cases there were better diagnostic clues through histological examination of bone mar-row particularly in assessing the cellularity, degree of fibrosis, extent of blast infiltration, percentage of infla-mmatory cells, dysplastic changes and residual haema-topoiesis. All these features were better noted in histo-logical examination of core biopsy.<br /><br />Conclusion:  The histological examination provided information additional to that provided by aspirate smears about the bone marrow changes in AML and suggested that some of the features may also have pro-gnostic significance in addition to diagnostic impor-tance.<br /><br />Key Words:  Acute myeloid leukemia, Bone marrow biopsy, bone marrow histology.<br /><br />}, number={1}, journal={Annals of King Edward Medical University}, author={Younas, Usman and Saba, Kanwal and Bukhari, Mulazim Hussain and Naeem, Samina}, year={2011}, month={Sep.}, pages={5} }